http://metadb.riken.jp/metadb/db/SciNetS_ria61i <div><img src="/dbfiles/SciNetS_ria61i/PSC_Logo1.png" width="65" height="69"><img src="/dbfiles/SciNetS_ria61i/P_NECSoft_horizontal.png" width="240" height="46"></div><div style="clear: both; -moz-border-radius: 10px 10px 10px 10px; padding: 10px; border: 3px double rgb(178, 178, 128); width: 800px; background: none repeat scroll 0% 0% rgb(255, 255, 238);"> We collected each full-length cDNAs prepared in RIKEN into an almost equimolarratio to generate plant expression library. In this library each full-lengthcDNA is located under CaMV35S promoter and TMV omega sequence for properprotein production. As a starting material we have collected around 10,000independent full-length cDNAs. By Agrobacteria inplanta transformation methoddeveloped in Arabidopsis we could transfer T-DNA region of the plant expressionlibrary into <em>Arabidopsis</em>. By repeating this transformation procedure we havegenerated around 30,000 independent <em>Arabidopsis</em> transgenic lines overexpressingfull-length cDNAs (FOX <em>Arabidopsis</em> mutant lines). For detail method of thelibrary construction, please refer to <a href="http://www3.interscience.wiley.com/journal/118565276/abstract?CRETRY=1&amp;SRETRY=0">Ichikawa et al., The Plant Journal (2006)45, 974-985</a>. All contents in this database have been constructed bycollaboration with <a href="http://www.necsoft.com">NEC Soft, Ltd</a> (VALWAY Technology Center, NEC Soft, Ltd,Tokyo, Japan).</div> <div><img src="/dbfiles/SciNetS_ria61i/PSC_Logo1.png" width="65" height="69"><img src="/dbfiles/SciNetS_ria61i/P_NECSoft_horizontal.png" width="240" height="46"></div><div style="clear: both; -moz-border-radius: 10px 10px 10px 10px; padding: 10px; border: 3px double rgb(178, 178, 128); width: 800px; background: none repeat scroll 0% 0% rgb(255, 255, 238);"> We collected each full-length cDNAs prepared in RIKEN into an almost equimolarratio to generate plant expression library. In this library each full-lengthcDNA is located under CaMV35S promoter and TMV omega sequence for properprotein production. As a starting material we have collected around 10,000independent full-length cDNAs. By Agrobacteria inplanta transformation methoddeveloped in Arabidopsis we could transfer T-DNA region of the plant expressionlibrary into <em>Arabidopsis</em>. By repeating this transformation procedure we havegenerated around 30,000 independent <em>Arabidopsis</em> transgenic lines overexpressingfull-length cDNAs (FOX <em>Arabidopsis</em> mutant lines). For detail method of thelibrary construction, please refer to <a href="http://www3.interscience.wiley.com/journal/118565276/abstract?CRETRY=1&amp;SRETRY=0">Ichikawa et al., The Plant Journal (2006)45, 974-985</a>. All contents in this database have been constructed bycollaboration with <a href="http://www.necsoft.com">NEC Soft, Ltd</a> (VALWAY Technology Center, NEC Soft, Ltd,Tokyo, Japan).</div> FOX Hunting FOX Hunting Imported from SciNetS Imported from SciNetS http://metadb.riken.jp/metadb/db/SciNetS_ria61i